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This paper is designed based on USB2.0 interface RS485 signal emulator. It is through the terminal application software to set serial communication parameters and send the data frame format, and the signal simulator developed within the loop feedback signal is about the actual data sent through the feedback loop back to the terminal application software and displayed. This paper details the USB interface chip FT245R the working principle and application platform in the MFC dynamic link library called FT245R interface function terminal application software design.